Week 5: Measurement and Imaging

From a chemical point of view, proteins are by far the most structurally complex and functionally sophisticated molecules known.
--- Molecular Biology of the Cell, 5th

What about this week on Measurement and Imaging?

Evan Daugharty (founder of ReadCoor from George Church'lab at Harvard) gave us a overview of analytical tools for synthetic biology.

Principles of Measurement & Imaging

Part I: Imaging using FoldScope

Part II: Design of smFISH / Spatial Sequencing Assay

Part III: smFISH Image Analysis

Reference

Requirement Background & Homework-Related Papers
• 2014. Highly multiplexed three-dimensional subcellular transcriptome sequencing in situ. Science
• 2015. Expansion Microscopy. Science
• 2010. RNA: state memory and mediator of cellular phenotype. Trends in cell biology
• 2014. Foldscope: Origami-based paper microscopy. PLose ONE
• The RNA Revolution. ACD Whitepaper