Week 4: Next Generation Synthesis

From a chemical point of view, proteins are by far the most structurally complex and functionally sophisticated molecules known.
--- Molecular Biology of the Cell, 5th

What about this week on Next Generation Synthesis?

Joseph Jacobsonfrom MIT gave us a overall introduction on DNA synthesis and its evolution. Then, the founder of Twist Bioscience Emily LeProust talked about a little history of DNA synthesis, the technology Twsit use and their new business plan.

Into the world of DNA manipulation

We will use mUAC and

mUAC_plate_view mUAC_plate_view mUAC_plate_view mUAC_plate_view mUAC_plate_view mUAC_plate_view assembly_workflow

Primer Design and Fragment Assembly

Remote Cloning

Reference

  • 2018. Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. PLOS ONE